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1、咸宁学院本科毕业设计（论文）：全自动生化分析仪的维护与保养 0 毕业论文（设计） 外文翻译 译文题目 :生化的分析者 将 Olli C + D 使用在朝派酶学一年后的评价 学生姓名： 吕锦阳 学 号： 050921060 专 业： 生物医学工程 方 向： 医疗器械 指导教师： 但汉久 2008 年 12 月 29 日 咸宁学院本科毕业设计（论文）：全自动生化分析仪的维护与保养 1 Evaluation of the Olli C + D biochemical analyser after over a year of use in enzymology G. Baraton, D. Graf

2、meyer, A. Capuano, L. Richard and J. Sofia. Laboratoire automatisde Biochimie Clinique, (Professeur J. Bertrand), HopitalEdouardHerriot, Place dArsonval, 69374 Lyon Cedex 2, France To meet todays clinical requirements, clinical biochemistry laboratories must be increasingly automated. The required c

3、riteria for an automatic analyser are reliability； the ability to change chemical reactions easily and quickly； rapid determinations and low analysis cost. It appears that the Olli C + D parallel analyser (Kone Oy instrument division Espoo, Finland) answers these criteria. A report on the Olli C sys

4、tem has already been published by Puuka and Pukka 1. This evaluation, using the C + Dsystem, deals with its intensive and daily use in enzymology over a period lasting more than one year. Description The instrument is a parallel, multichannel, photometric analyser. The different phases of determinat

5、ion are performed in batches of 24 samples including blanks, standards and controls. The rate of analysis is 700 tests per hour with end point determinations of 360 kinetic measurements per hour reaction time is 21A minutes. The analyser comprises three independent units, the sample diluter/processo

6、r, photometer (each controlled by microprocessor) and incubator. The sample change is performed by hand using disposable cuvettes in a thermostatedblock. The Olli D Diluter measures 350 x 1100 x 740 mm and weighs 70 kg. It is made up of a dispensing tray with 24 cuvette blocks which fit in the Olli

7、C photometer, eight reagent vessels, one block with 24 serum vials, an eight tip dispensing head and a digital display and keyboard. The diluter can be programmed by hand or by tape cassette for 30 analyses； it is possible to add serum and up to eight reagents distributed in one or several runs. Eac

8、h analysis programme follows the sequence:l. Attain reaction temperature (25, 30, 37C). 2. Measure reagent (5 to 1000/al by steps of 1/.tl) and position. 3. Measure and add sample. 4. Mix(5 to 180 seconds). The photometer measures 330 x 520 x 740 mm and weighs 48 kg. It comprises a computer, a therm

9、al pri_nter and a thermostated measuring chamber (25, 30, 37C). The light source is a zenon lamp. A quartz optic fibre system allows the simultaneous measurement of 24 cuvettes. Filters have a bandwidth of 8 to 15 nm. The smallest readable volume is 500 /al for end point determinations, and 300/1 fo

10、r kinetic determinations. The photometer can be programmed by hand or by tape cassette for 15 analyses which may be made in end point mode, with or without blank measurement, or in kinetic mode, with or without reagent or serum blanks. Kinetics determinations are made using linear regression of 12 t

11、o 24 measurements in to 10 minutes. The results are printed with the name and units of the programmed analysis. Error messages are printed； eg, if the chosen initial absorbance limits have been exceeded or if there is non-linearity of reaction. In addition, it is possible to have printed the twelve

12、or 24 absorbance measurements used for the calculation of the activity of each determination. The Olli incubator can handle four thermostated blocks. Each block contains one heating element and temperature regulation is maintained via a water circulating system. The incubation 咸宁学院本科毕业设计（论文）：全自动生化分析

13、仪的维护与保养 2 period is determined by a timer and each block incubation time is terminated with an audible alarm. Material and metlods The evaluation was carried out according to the rules suggested by the French Society for Clinical Biology (SFBC) 2, 3. Dilution and measurement Accuracy, within-block a

14、nd day-to-day, was tested with a solution of drawing ink (Mars 745 from Staedler Germany) prepared as follows: 1.7 ml of ink as supplied was dissolved by gentle stirring in 1000 ml of distilled water and filtered in a buchner funnel through silicone paper. The solution was preserved with Merseptyl a

15、nd stored at +4C. Dilutions were made by the Olli D processor into Olli arcylic cuvettes and absorbances were read on the Olli C photometer at 405 nm. Measurements were verified by manual dilution followed by a reading using a Miniken spectrophotometer (Coultronic). Control of temperature The contro

16、l of the temperature variations between 25 and 37C was tested by measuring the absorbance of a paranitrophenol solution, (15 mg in 1000 ml of Tris HCL 0.2 M, pH 6.8) at 405nm, Naudin et al 4. The absorbances obtained at the chosen temperature were compared with those obtained using four thermostated

17、 analysers； Miniken (Coultronic),Cobas BIO (Roche,) PA 800 (Vitratron), ACP 5040 (Eppendorf) On the Olli C, the time needed to reach the set temperature of 30C or 37C with an analysis volume of 550 ul(contained in the acrylic cuvette in the thermostated block)was followed by noting the decrease in t

18、he absorbance of the paranitrophenol solution. The temperatures of cuvette contents were also monitored with a Metrix temperature probe (HA 1159). Optical performance This was tested according to the procedure of Rand 5.Linearity and precision were tested using the following kits:UV-Trol (Biomerieux

19、 France) and Holnicob (Biotrol France). Method All analyses were performed at either 30C or 37C. The instrument settings were those recommended by the manufacturer.Glutamic oxaloacetic transaminase (SGOT) and pyruvic transaminase (SGPT) were measured by the Wroblewski method at 37C or by the recomme

20、nded method of the SFBC at 30C with the Biomerieux kits. Alkaline phosphatase (ALP) was measured following the recommended procedure of the German Society of Clinical Biochemstry (DGKC) at 37C. Accuracy, within day and day to day, and carryover were tested using low, medium and high concentration co

21、ntrol sera spiked where necessary with animal enzymes. These control sera were furnished by a local association operating in biological measurement quality control. (Pro Bio. Qual, Lyon, France). Results and discussion Accuracy and precision of the Olli D sample processor The results are presented i

22、n Table 1. Precision expressed as the coefficient of variation for 24 determinations was never greater than 0.925% for the most important dilution (10 of drawing ink in 510 1 of water). The measured absorbances varied directly with the amount of drawing ink added and were indistinguishable from those obtained by manual dilution. Day-to-day precision was 0.609% for the 1/12 dilution used routinely for SGOT and SGPT and 0.925% for the 1/30 dilution used routinely for ALP.