外文翻译---数据采集：简介

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1、 附录 A 英文文献原文 Data Acquisition: An Introduction Bruxton Corporation This is an informal introduction digital data acquisition hardware. It is primarily directed towards assisting in the selection of appropriate hardware for recording with the Acquire program. Overview In principle, data acquisition h

2、ardware is quite simple. An A/D converter delivers a sequence of values representing an analog signal to an acquisition program. In practice, selecting and properly using data acquisition hardware is more complex. This document provides an informal introduction to the topic. . Many of the examples a

3、re taken from patch-clamp recording. This technique requires accurate acquisition of low-level signals (picoamperes) with bandwidth in the audio range (up to 10kHz). Background A data acquisition system converts a signal derived from a sensor into a sequence of digital values. The sensor is connecte

4、d to an amplifier, which converts the signal into a potential. The amplifier is in turn connected to a digitizer, which contains an A/D converter. The digitizer produces a sequence of values representing the signal. Signal Source The source of most signals to be digitized is a sensor, connected to a

5、n amplifier with appropriate signal conditioning. The amplifier delivers an electrical signal. This signal is then digitized using an A/D converter. For patch-clamp recording, the sensors are solution filled pipettes. The pipette is connected to a patch-clamp amplifier that converts the voltage at t

6、he pipette or the current through the pipette to a high-level signal. By convention, the full-scale output range of a patch-clamp amplifier is 10V, matching the range of common instrumentation quality digitizers. Digitizer A digitizer converts one or more channels of analog signal to a sequence of c

7、orresponding digital values. The heart of a digitizer is an A/D converter, a device that samples an analog signal and converts the sample to a digital value. C o n t e n t s B a c k g r o u n d 1 F r o m S e n s o r s t o S i g n a l s 2 F r o m S i g n a l s t o S a m p l e s 2 F r o m S a m p l e

8、s t o C o m p u t e r 4M e a s u r e m e n t A c c u r a c y 5s e n s o rA m p l i f i e r D i g i t i z e r+ 3 . 2 5 0+ 3 . 1 0 0+ 2 . 5 0 0+ 1 . 7 4 5+ 0 . 9 8 5For example, for recording from a single ion channel, the digitizer might determine the output of the patch clamp amplifier once every 50

9、ms and provide the resulting value to the computer. Sampling Theorem The purpose of data acquisition is to analyze an analog signal in digital form. For this to be possible, the sequence of values produced by a digitizer must represent the original analog signal. The sampling theorem states that thi

10、s is the case. The sampling theorem states that an analog signal can be reconstructed from a sequence of samples taken at a uniform interval, as long as the sampling frequency is no less than double the signal bandwidth. For example, assume a signal contains frequencies from DC (0Hz) to 10kHz. This

11、signal must be sampled at a rate of at least 20kHz to be reconstructed properly. As a practical matter, the sampling rate should be several times the minimum sampling rate for the highest frequency of interest. For example, to resolve a 10kHz signal, a minimum sampling rate of 20kHz is required, but

12、 a sampling rate of 50kHz or more should be used in practice. Control Most of this discussion is about digitizing analog signals for a computer. In many cases, a computer also produces analog control signals. For example, in patch-clamp experiments involving voltage-gated ion channels, the computer

13、is frequently used to produce an electrical stimulus to activate the channels. These control signals are produced using a D/A (digital to analog) converter. From Sensors to Signals Many signal sources consist of a sensor and an amplifier. The amplifier converts the output of the sensor into the sign

14、al to be digitized. Preamplifier Many instrumentation systems are built with a preamplifier located as close to the sensor as possible. A separate amplifier converts the preamplifier output to a high-level signal. Placing the preamplifier close to the sensor reduces noise, by allowing the signal to

15、be amplified before being sent over a cable. Since physical space near the sensor is limited, the preamplifier is as small as possible, with the bulk of the electronics being located in the amplifier. For example, in a patch clamp setup, the sensor is a solution-filled pipette, the preamplifier is t

16、he head stage, and the amplifier is the patch-clamp amplifier itself. Signal Conditioning Many sensors deliver signals that must be transformed before they can be digitized. For example, a microelectrode pipette may be used to measure current, while the digitizer measures potential (voltage). The patch clamp amplifier provides a current-to-voltage amplification, usually measured in mV of output per pA of input. This transformation of the sensor signal is called signal H e a d s t a g e A m p l i f i e rM i c r o e l e c t r o d e