1、外文标题： Identification of appropriate reference genes for RT-qPCR analysis in Ziziphus jujuba Mill 外文作者： Chunmei Zhang, Jian Huang, Xingang Li 文献出处 : Scientia Horticulturae , 2015 , 197 :166-169 英文 3007单词， 16798字符，中文 4692汉字。 此文档是外文翻译成品
2、，无需调整复杂的格式哦！下载之后直接可用，方便快捷！只需二十多元。 原文： Identification of appropriate reference genes for RT-qPCR analysis in Ziziphus jujuba Mill Chunmei Zhang, Jian Huang, Xingang Li Abstract: Reverse transc ription quantitative real-time PCR (RT-qPCR) is the most w ide ly used method to evaluate the ex
3、pression levels of mRN A, and the stability of referenc e genes is cruc ial for c orrec t evaluation of RT-qPCR data. In the present w ork, 11 candidate referenc e genes w ere investigated for their expression levels at six fruit developmental stages and among four tissues and ve genotypes of
4、Ziziphus jujuba Mill. The geNorm, Normnder and Bestkeeper software programs were applied to estim ate the stability of the 11 referenc e genes. The results show ed that UBQ, ACTIN, CYP and ACTIN9 for the fruit developmental stages, UBQ2 and UBQ &
5、nbsp;for the different tissues, UB Q2, GAPD H, ACTIN9 and EF1 for the different genotypes, and UBQ, ACTIN9, UBQ2 and CYP for all samples evaluated w ere stably expressed. Additiona lly, the relative GME expression level w as used to validate the referenc e genes. The ndings may enable more acc
6、urate assessments of gene expression in Z. jujuba Mill. Keyw ords Ziziphus jujuba Mill, Referenc e genes, Gene expression, Quantitative real-tim e PCR Introductio n Chinese jujube, or jujube (Ziziphus jujuba Mill.), a native fruit tree from China, is one of the most im portant spec ies in the fam -
7、ily Rha mnac eae. With ric h nutritional and medic ina l properties (Qu and Wang, 1993), the jujube c ultivation area has reac hed 2 million hm2 and is also inc reasing around the w orld (Liu et al., 2013a). As an important ec onomic fruit tree, it is nec essary to understand the molec ular basis of i
