1、PDF外文：http:/ Biological effects of the Magnetic Stimulation on the Toad Heart Hongwei Lei,Yuyuan Du,Yina Li,Chunling Liu,Xu Wan 2007 IEEE/ICME International Conference on Complex Medical Engineering-CME2007 Institute of Biomedical Engineering, Northeastern University, Shenyang, Liaoning 110004
2、, P.R.CHINA Abstract-We stimulated the exposed toad heart by a low frequency and high energy magnetic. By analyze the data of this experiment, it shows that the pulsating of the weak toad heart would make change after stimulated by magnetic. Weak heartbeat strengthened, the single peak curve would b
3、ecome the two peaks curve with atria wave and ventricle wave after the magnetic stimulation. But the cycling of rhythmic pulsatile curve of toad doesn't change. I. INTRODUTION All life forms have magnetism. All kinds of magnetic field would have some effects on the configuration and activities o
4、f life forms that whichever environmental magnetic, additional magnetic or inside magnetic of organism. The biologic effects are related to the characteristics and the intension of the magnetic field, as well as the species and the tissues of the life forms. The experimentation showed that magnetism
5、 stimulation in some range would control the growth of rat tumour, whatever they are in or out the body. Much more they can induce the cancer cells dead.30mT magnetic stimulation would increase the content of NO in the liver and the kidney.Magnetic also can improve the activity of some enzyme and pr
6、omote the regeneration of nerve tissue.Cell would increase, the bones would be concrescence, the scar would be rehabilitate.The blood rheology and blood cell number both of human and rat would change obviously, DI the blood mucosity would be low. Heart is the most important apparatus of life. It pul
7、sates day and night. Heart once stop pulsating, the life for danger.Numerous scholar pays attention to the role of magnetic field.But they just studied the effects of magnetic stimulation of the heart pacemaker. The experiments about direct effects of stimulate heart by magnetic is very few. The toa
8、ds are our experiment animals.We stimulated and noted by the magnetic stimulation equipment and the noted equipment of pulsatile curve made by ourselves. Analyze the results. II. STUFF A.Experiment equipments: magnetic stimulation equipment； (magnetic intension 8-10T, impulse width 150ms,maximal sti
9、mulation frequency 5Hz)； software of noted pulsatile curve (made by ourselves)； cardiomuscular transducer； Ringer.Sol ； Batrachia instruments； clip of frog heart； cotton thread； burette. B.Experiment animals: toads. III. METHOD A. Destroy the brain and the spinal cord of the toad by styl
10、et:Penetrate into the occipital aperture upright with stylet,destroyed the brain upwards, take back the stylet and destroy the spinal downwards. If the limb of toad were relaxed, it showed that the brain and spinal were destroyed completely. B. Expose the toad heart: Make the toad lying on its back
11、on the winding center. The magnetic aspect is upright through the toad heart.Cut the ventral skin of toad, snip the breastbone,expose the rat heart. Nip the heart tip by clip carefully. Make the cotton thread tied with the clip of frog hear the linked with the cardiomuscular transducer. Do not make
12、the toad heart leave thorax, or it would disturb the experiment results. C.Noted the result:Connect the cardiomuscular transducer with the computer. Take notes the curve of toad heart without giving the stimulate of magnetic field D. After three minutes, noted the weak pulsatile curve. E . Make the
13、magnetic intension 10T, electricize 10s.Stimulate the toad heart and record the pulsatile curve. IV. RESULTS The abscissa of cardiac rhythmic pulsatile curve is time, the ordinate is constriction power. Take notes for the pulsatile curve of toad heart that exposed just.We can know the rhythmic pulsa
14、tile cycle of the toad heart is 1.5s from fig 1 which show the cardiac rhythmic pulsatile curve of the toad which was exposed the heart just now. There are two waves in each cycle, one is atria wave, the other is ventricle wave. The atria wave is 0.5s and the ventricle wave is 1.0s. The constriction
15、 power of atria is less than that of ventricle. The amplitude of constriction power of ventricle is the 2 times of the atria. Fig. 4.1. It is rhythmic pulsatile curve of the toad without magnetic stimulation. The constriction power of toad heart would become weaker after the toad heart was exp
16、osed for a while. At the same time,atrium wave and ventricle wave can not be already distinguished. Heart contracting amplitude were reduced obviously, do not go to the half of original atrium wave. The rhythmic pulsatile cycle of the toad heart is still 1.5s. Fig. 4.2. It is the weak pulsatil
17、e curve of toad without magnetic stimulation. But we can distinguish the atria wave and the ventricle wave again after giving the toad heart a magnetic stimulation on following picture. And the amplitude of ventricle waves is more than that of the single wave. The rhythmic pulsatile cycle of the toa
18、d heart is still 1.5s. There were six toads as experiment animal in our experiment.After exposing heart a time, the rhythmic pulsatile curve all became single peak curve. Stimulate them when the single amplitude was 0.95. Noted the data and analyze them. Following is the pulsatile curve of the six toads recorded which were stimulated by magnetic field.
